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Right, Env-specific IgG ASCs measured from splenocytes at week 5. (C) Env-specific serum IgG titers were measured at weeks 5, 6, and 8. (D) Week 8-to-week 5 ratio of Env-specific serum IgG titers.

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Briefly, aliquots of 1.25 to 5.0 × 105 spleen and BM cells were plated in triplicate in NP-BSA–precoated 96-well PVDF membrane plates (Millipore) and incubated overnight at 37 °C in 5% CO2.

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